Journal: International Journal of Molecular Sciences

Article Title: Proteomic Analysis and Expression of Selected Genes During the Early Somatic Embryogenesis of Jatropha curcas L.

doi: 10.3390/ijms26136384

Figure Lengend Snippet: Representative 2D IEF-SDS-PAGE protein profiles of J. curcas somatic embryos. The analysis was carried out using ImageMaster TM 2D Platinum software version 7. Molecular masses (kDa) are indicated on the left, and isoelectric points (pI 4–7) are provided above the gel image. ( a ) Protein profile of pre-globular somatic embryos with an average of 654 ± 41 detected spots, and ( b ) protein profile of globular-stage somatic embryos with 552 ± 30 detected spots as indicated by a red cross. The blue and red circles on the gel indicate the protein spots identified through mass spectrometry; the data obtained are presented in . Red circles correspond to unique protein spots detected exclusively at the globular stage, while blue circles indicate protein spots that were at least 1.5-fold more abundant in the embryogenic stage than in the other stages. Numbers correspond to spots identified with ImageMaster TM 2D Platinum version 7. First-dimensional isoelectric focusing was performed using 11 cm IPG strips loaded with 200 µg of total protein. Second-dimension separation was carried out using 12.5% SDS-PAGE polyacrylamide gels (CRITERION Cell, Bio-Rad Laboratories, Hercules, CA, USA). Gels were stained with Sypro Ruby (Bio-Rad Laboratories, Hercules, CA, USA).

Article Snippet: The second electrophoretic dimension was performed on 12.5% CRITERION TM precast polyacrylamide gels (Bio-Rad Laboratories, Hercules, CA, USA).

Techniques: SDS Page, Software, Mass Spectrometry, Staining